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KMID : 1123920090230030673
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Korean Journal of Oriental Physiology and Pathology
2009 Volume.23 No. 3 p.673 ~ p.677
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Anti-inflammatory Effects of Gelidium amansii in RAW 264.7 Macrophages
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Choi Won-Sik
Kim Young-Sun
Lee Sang-Hyun
Chai Kyu-Yun
Lee Young-Haeng
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Abstract
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In order to verify the anti-inflammatory effects of Gelidium amansii, RAW264.7 macrophages were incubated with the extract of 70% ethanol solution (Ex), and activated with the endotoxin lipopolysaccharide (LPS). Ex inhibited the expression of the pro-inflammatory enzymes, including inducible nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2), and the production of iNOS-mediated NO and COX-2-mediated prostglandin () production in a dose-dependent manner. Ex also reduced the release of the pro-inflammatory cytokines, including tumor necrosis factor- (TNF-), interleukin-1 (IL-1) and IL-6 in LPS-activated macrophages, The observed anti-inflammatory effects of Ex was associated with inactivation of the nuclear factor (NF-) that mediates the induction of iNOS, COX-2, TNF-, IL-1, and IL-6. Further studies showed that Ex inactivated NF- through inhibition of phosphorylation of the inhibitory (), Taken together, these results suggest that Gelidium amansii exerts anti-inflammatory effects by inhibiting the expression of pro-inflammatory enzymes and the secretion of pro-inflammatory cytokines via inactivation of NF- and/or .
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KEYWORD
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Gelidium amansii, macrophage, pro-inflammatory enzyme, pro-inflammatory cytokine, anti-inflammatory effect
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